By Ramanathan Nagarajan (Eds.)
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ACS Symposium Series; American Chemical Society: Washington, DC, 2012. ch002 Figure 13. The alignment of virus particles inside the capillary tube generates striped patterns. 1 mg/mL TMV solution in capillary tube. (b) 3D atomic force microscopy height image of aligned TMV particles. (c) An enlarged region of highlighted box in (b). (d) Fluorescence images of rat aortic smooth muscle cells cultured inside the capillary without stripe patterns, (e) the same cells cultured inside the capillary tubes with patterned surface.
Actin is labeled with green fluorescent phalloidin, and the nuclei with 4′,6-diamidino-2-phenylindole (DAPI). Scale bars: 100 μm. Reproduced from ref (153) by permission of The Royal Society of Chemistry. Copyright (2008). Similar to our previous report, when 3T3 and BHK cells were cultured for 24 h on the aligned M13 films, the majority of the cells displayed elongated, spindle-like morphologies. The cells grown on coverslips were randomized with stellate morphologies (Figure 10a, d). ; ACS Symposium Series; American Chemical Society: Washington, DC, 2012.
Figure 9. Optical images using an inverted phase contrast microscope. (a) Optical image of NIH-3T3 cells cultured on an aligned M13 thin film by the slow dragging method. (b) Fluorescence image of NIH-3T3 cells. (c) Optical image of CHO cells cultured on an aligned M13 thin film by the slow dragging method. (d) Fluorescence image of CHO cells . β-actin is labeled with green fluorescent phalloidin, and the nuclei with 4′,6-diamidino-2-phenylindole (DAPI). Scale bars: 100 μm. Reproduced from ref (153) by permission of The Royal Society of Chemistry.