By Maria B. Dainiak, Ashok Kumar, Igor Yu. Galaev, Bo Mattiasson (auth.), Ashok Kumar, Igor Yu Galaev, Bo Mattiasson (eds.)
M. B. Dainiak, A. Kumar, I.Y. Galaev, B. Mattiasson: equipment in mobilephone Separations.-
S.F. Ibrahim, G. van den Engh: circulation Cytometry and phone Sorting.-
A.A. Neurauter, M. Bonyhadi, E. Lien, L. Nøkleby, E. Ruud, S. Camacho, T. Aarvak: phone Isolation and growth utilizing Dynabeads®.-
J. Hubble: Affinity Adsorption of Cells to Surfaces and methods for mobilephone Detachment.-
M.B. Dainiak, I.Y. Galaev, A. Kumar, F.M. Plieva, B. Mattiasson: Chromatography of dwelling Cells utilizing Supermacroporous Hydrogels, Cryogels.-
R.E. Nordon, S. Craig: Hollow-Fibre Affinity mobile Separation.-
J.M.S. Cabral: telephone Partitioning in Aqueous Two-Phase Polymer Systems.-
M. Kamihira, A. Kumar: improvement of Separation approach for Stem Cells.-
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Extra resources for Cell Separation: Fundamentals, Analytical and Preparative Methods
Clones displaying a certain phenotype can be sorted, their DNA PCR-ampliﬁed, and sequenced to identify sequences of note. In the case of Saccharomyces cerevisiae with a genome size of approximately 14 Mbp, 100 bp fragments could be generated and placed upstream of a reporter construct, creating a library on the order of 105 clones. The entire genome could be scanned to 10X coverage for the occurrence of a particular genetic event in a matter of minutes using high-speed cell sorters. As an example, a study by Barker et al.
6) . Several recent reviews outline other ingenious applications for ﬂow cytometry and cell sorting in the arena of biotechnology as well as discuss changes in the technology behind these instruments [1, 3, 83–91]. F. Ibrahim · G. van den Engh Fig. 6 Individual E. Coli sorted directly on to an agar plate based on expression of GFP vs. dsRED and allowed to incubate for 36 h. Single bacterial cells were sorted directly on to the agar plate, alternating rows of cells expressing GFP and dsRed. After incubation, discrete pure colonies of cells expressing the respective ﬂuorescent protein are seen.
Goodell MA, Brose K, Paradis G, Conner AS, Mulligan RC (1996) Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med 183:1797–1806 49. Wognum AW, Eaves AC, Thomas TE (2003) Identiﬁcation and isolation of hematopoietic stem cells. Arch Med Res 34(6):461–475 50. Ivanova NB, Dimos JT, Schaniel C, Hackney JA, Moore KA, Lemischka IR (2002) A stem cell molecular signature. Science 298:601–604 51. Eliopoulos N, Al-Khaldi A, Beausejour CM, Momparler RL, Momparler LF, Galipeau J (2002) Human cytidine deaminase as an ex vivo drug-selectable marker in gene modiﬁed primary bone marrow stromal cells.