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Extra info for Cell Biology: A Comprehensive Treatise. Gene Expression: The Production of RNAs

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Coli and those of eukaryotes revolve around their multiplicity, albeit limited, and the fact that one copy of each of 16 S and 23 S rRNA gene is present in the transcription unit. ; The possession of the 5 S rRNA gene and "spacer" tRNA genes n the transcription unit, and the absence of tandem repetition, contrast with the eukaryotic rRNA gene organization. C. , 1968), the genes for 5 S R N A (5 S D N A ) are present in about 25,000 copies per haploid genome in a nonnucleolar location (Brown and Weber, 1968).

Those families that reasso­ ciate at low C y values have properties similar to diverged satellite DNA's, and may well have had their origins there (Southern, 1974). , 1974). C. Single-Copy Sequences A single-copy sequence is, by definition, one that occurs only once in each haploid genome. From an experimental point of view a single-copy sequence is one that reassociates with a CVi/ 2 value expected for a se­ quence complexity equal to that of the size of the genome. In practice, it is difficult to estimate exactly the proportion of the genome that is unique, since it is highly dependent on the conditions of reassociation.

The unit contains both a transcribed region and a nontranscribed region. The former is of constant length and contains the 18 S and 28 S r R N A genes and transcribed spacer. The nontranscribed spacer is of variable length. EcoRl sites are indicated by the arrows. The numbers refer to lengths in nucleotide pairs of different segments. For fuller explanation and the sources from which this was compiled, see text. Analysis of ribosomal D N A fragments from Xenopus which had been cloned in bacterial plasmids shows that the nontranscribed spacer region is itself composed of a repeating 50 nucleotide pair sequence.

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