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E. It inhibits viral protein synthesis It inhibits RNA synthesis It inhibits viral DNA polymerase It stimulates DNA provirus production It inhibits viral reverse transcriptase 26. Which of the following enzymes can polymerize deoxyribonucleotides into DNA? a. b. c. d. e. qxd 18 11/14/01 10:36 AM Page 18 Biochemistry and Genetics 27. Which of the following statements correctly describes the recombinant DNA tool known as plasmids? a. b. c. d. e. They are found more commonly in viruses than in bacteria They are single-stranded circles They sometimes enhance bacterial susceptibility to antibiotics They sometimes enhance bacterial resistance to antibiotics They are too small to be useful as vectors for the cloning of mammalian DNA segments 28.
Sack, pp 3–29. ) Since both strands of parental DNA serve as templates for the synthesis of new DNA, it appears that DNA synthesis must be 5′ to 3′ for one daughter strand and 3′ to 5′ for the other daughter strand at the replication fork. Despite the apparent need for 3′ to 5′ synthesis, all DNA polymerases and repair enzymes can only synthesize DNA in the 5′ to 3′ direction. The apparent contradiction is solved by understanding that one strand of DNA is synthesized continuously in the 5′ to 3′ direction while the other strand is made up of small fragments known as Okazaki fragments.
Which of the following is the most accurate description of DNA fingerprinting? a. DNA can be isolated from blood, skin, or sperm and analyzed for variable patterns of restriction fragments arising from tandemly repeated sequences (microsatellites) b. DNA is copied from blood, skin, or sperm RNA using reverse transcriptase and analyzed for the pattern of complementary DNAs c. DNA is isolated from blood, skin, or sperm and its fragment size distribution is analyzed by gel electrophoresis d. DNA is isolated from blood, skin, or sperm and hybridized with probes from the HLA locus to visualize HLA gene patterns e.