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Thus the V, V*, V** set and the P, P*, P** set each represents a heterogeneous terminal fragment from the leftward or rightward orientation of one segment of the genome and the G, G*, G** and H, H*, H** sets are the equivalent species in the Xbal digests. The difference in size between the 11-14 kb Xba-G and H species and the 5-8 kb P amd V complexes accounts for the homology of the XbaI-(G,H) probe to the internal HindIII-T and -F fragments. Other experiments revealed that the opposite end of CMV DNA gives rise to the Xbal-L and HindIII-K terminal fragments which cross-hybridize.
TAMASHIRO AND DEBORAH H. SPECTOR These viruses have been associated with a variety of diseases and may have oncogenic potential (see 2,3,4, and 5 for reviews). An infection with HCMV is most often asymptomatic; however, the exceptions are severe enough to make this a medically significant virus. HCMV infection is the most frequent perinatal infection of newborns and involves approximately 1% of all live births. Although only a fraction of these infants exhibit symptoms of generalized cytomegalic inclusion disease, (CMID), several follow-up studies have indicated that a large number of presumably "asymptomatic" patients have suffered some degree of sensorineural hearing loss, mental retardation, and other neurological sequelae (6, 7,8,9).
2. A MODULAR THEORY OF VIRUS EVOLUTION 17 The Role of Homologous Recombination. e. the virus) be properly regulated regardless of which particular modules are present for each biological function. This assurance is intrinsic to the way in which these phages are regulated and by the method of interchange which is homologous recombination. The regulation of the lambdoid bacteriophages (including, of course P22) is at the level of transcription, through a cascade of regulators each of which controls transcription at sites immediately adjacent to itself.