By Nord F F (ed)

Advances in Enzymology and similar components of Molecular Biology is a seminal sequence within the box of biochemistry, providing researchers entry to authoritative reports of the newest discoveries in all components of enzymology and molecular biology. those landmark volumes date again to 1941, offering an unmatched view of the old improvement of enzymology. The sequence bargains researchers the newest knowing of enzymes, their mechanisms, reactions and evolution, roles in advanced organic strategy, and their program in either the laboratory and undefined. every one quantity within the sequence good points contributions through top pioneers and investigators within the box from around the globe. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.

With its wide selection of issues and lengthy old pedigree, Advances in Enzymology and similar components of Molecular Biology can be utilized not just through scholars and researchers in molecular biology, biochemistry, and enzymology, but in addition through any scientist attracted to the invention of an enzyme, its houses, and its purposes.


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Extra info for Advances in Enzymology and Related Areas of Molecular Biology, Volume 29

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Diagram of antiport system for solutes A and B carried aa XA and XB. The arrows show the direction of the processes when a gradient of A causes translocation of B, which is a t the same concentration on left and right. The single barbs indicate that each reaction is reversible. ” There are three chemical channels, correspondingto translocation of XA, XB, and X. X channel (increasingf x relative to f X A and f X B ) . In the special case that the carrier can move only when occupied by A or B, the X channel is closed (fx is infinite) and the coupling is absolute (dnA/dt d n B / d t = 0).

VI. The Difference between Primary and Secondary Translocation and the “Driving” of Transport by Metabolism. . . . . . . . . . ,. . . . . . .. . . . ... References. . . . . . 33 39 41 43 43 44 44 44 46 49 53 53 54 59 61 65 66 69 69 73 76 78 79 I. The Facilitation of Diffusion by Catalytic Carriers* The recent work on the conduction of oxygen through solutions of hemoglobin and myoglobin affords an elegant illustration of the *Abbreviations used : ADP and ATP, adenosine di- and triphosphates ; Pi, inorganic orthophosphate; Tris, tris(hydroxymethy1)amino methane; DNP, 2,4-dinitrophenol; CFCCP, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone; NAD, nicotinamide adenine dinucleotide; FAD, flavin adenine dinucleotide; CoQ, coenzymeQ; cyt, cytochrome; o/r, oxidoreduction.

3M) for the following sugars (in order of declining affinity) : D-mannose, D-galactose, D-xylose, L-arabinose, D-ribose, D-arabinose. On the other hand, the maximum velocity (Vmax) of translocation was the same, within experimental error. 1M for D-arabinose (53). Since V,,, is independent of the substrate, it seems that either the frictional coefficient for the translocation of the carrier-substrate complex is virtually independent of the substrate, or the translocation of the carrier-substrate complex is not the rate-limiting reaction.

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